Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility.
نویسندگان
چکیده
منابع مشابه
Study of mitochondrial membrane potential, reactive oxygen species, DNA fragmentation and cell viability by flow cytometry in human sperm.
BACKGROUND Sperm cell death appears to be a cause of male infertility. The objective of this study was to determine the most reliable method for the evaluation of sperm quality in semen samples during sperm preparation for IVF. METHODS Conventional analysis of semen samples was compared with several cytofluorometric methods detecting death-associated changes. Neat semen from infertile patient...
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In this study, we conducted a simultaneous analysis of sperm count and viability in rats by flow cytometry (FCM). Epididymal fluids were taken from the caudal epididymis of 12 to 13 week-old Sprague-Dawley rats. The fluids were weighed and mixed with Dulbecco's phosphate buffered saline (D-PBS). Propidium iodide, which can stain only dead sperm, was used to distinguish viable and dead sperm. Th...
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Objective(s): Increased levels of nitric oxide (NO) in the testicular veins of people suffering from varicocele have already been reported. However, the role of NO-synthase (NOS) isozymes and their inhibitors have not been extensively studied. We aimed to evaluate the inhibitory effects of aminoguanidine (AG), on sperm motility, vitality, and mitochondrial membrane potential (MMP) in varicoceli...
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In yeast the use of rhodamine 123 (Rh123) has been restricted to the evaluation of mitochondrial respiratory function including the discrimination between respiratory-competent and -deficient cells. This study describes the optimization and validation of a low-concentration Rh123 staining protocol for the flow-cytometric assessment of mitochondrial membrane potential (Delta Psi m) changes in wh...
متن کاملprotective effect of silymarin on viability, motility and mitochondrial membrane potential of ram sperm treated with sodium arsenite
background: sodium arsenite can impair male reproductive function by inducing oxidative stress. silymarin is known as a potent antioxidant.objective: this study was performed to investigate if silymarin can prevent the adverse effect of sodium arsenite on ram sperm viability, motility and mitochondrial membrane potential.materials and methods: epidydimal spermatozoa obtained from ram were divid...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1982
ISSN: 0022-1554,1551-5044
DOI: 10.1177/30.3.6174566